Preventive measures and customs in families not infected by COVID-19 during confinement

Despite the exuberant figures of those infected and deceased by COVID-19, there are families that were not infected. Objective: To describe the preventive measures and customs in families not infected by COVID-19 during confinement. Method: Descriptive qualitative research, 13 mothers from Ferrenafe with no member affected by COVID-19 participated. The data was collected through the semi-structured interview through telephone calls and processed manually, with thematic content analysis. Results: Four categories were obtained: a) Preventive measures when leaving the home: use of a mask, alcohol and social distancing, b) Preventive measures at home: handwashing, disinfection of the home and what enters, c) Restriction of family gatherings and use of social networks, d) Change of eating habits and use of home remedies. Conclusions: The families changed some customs or practices, complied with the confinement, social distancing, restricted family gatherings, used cloth masks, medicinal alcohol, and bleach. In addition, they increased the frequency of hand washing, home hygiene, healthy eating, the use of social networks, together with the use of home remedies commonly used to prevent or treat respiratory diseases.

Reference Method for the Care of Health Consultations of Older Adult Users Based on Chatbot, in a Context of Pandemic

The COVID-19 pandemic has marked a considerable event in the history of all countries, causing a high degree of mortality rate in older adults, in Peru health care for this group of users has become relevant. In this sense, the EsSalud Center for the Elderly (Social Health Insurance) in the town of Sicuani (CAM - Sicuani) needs to protect its population. This research consisted of proposing a reference method for health consultations based on chatbot, avoiding face-to-face consultations;for which a reference method was developed to design and develop the chatbot, in order to allow it to answer user queries online and immediately. For this purpose, information was obtained from CAM - Sicuani regarding the daily queries made by users. Information was also collected on the topics, questions and answers related to COVID-19, this from the websites of the World Health Organization (WHO), EsSalud, Ministry of Health (MINSA). On the other hand, there are not many research and projects related to the chatbot. A chatbot development method was developed based on Amir Shevat's book consisting of 4 phases;The first phase called use case definition and exploration, the second conversation scripts, the third design and testing and finally the development of the chatbot. Regarding the results, through the analysis of the metrics it is shown that the chatbot is efficient in response time with an average of 0.8 ms in the confusion rate, the chatbot responds in real time;for the performance of the chatbot, it was verified that it is flexible and optimal, since the capacity of the chatbot is adaptable;for the last metric, which is the rating of the chatbot, it was evaluated based on star rating;through a visual scale, where an average of users was randomly selected for the chatbot tests in this first initial version, who were satisfied with a rating of 3 - 5 stars;With the chatbot, it contributes to having a significant improvement in the process of attending to queries, generating a positive attitude in users;In addition, CAM - Sicuani and its users will benefit from the chatbot in relation to minimizing costs and optimizing time, which is not subject to any alteration. We conclude that the chatbot in its initial version works efficiently, in addition this innovation will prevent people from attending these health consultation services in person, on the other hand the digital transformation is promoted and with this a competitive advantage is obtained. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

Functionality in healthcare workers with COVID-19 in Cali (Colombia)

Introduction: The COVID-19 pandemic has caused a high impact on the general population, healthcare workers have not been the exception, due to their constant exposure to patients positive for SARS-COV2. The evaluation of its functionality after the disease is an important issue. Objective(s): To describe the functional status of healthcare workers who survived COVID-19 in Cali (Colombia) Methods: Cross-sectional study that included healthcare workers who participated in the follow-up by the ambito laboral area of the Secretaria de Salud Publica of Cali;who suffered from COVID-19. The Spanish version of the Post COVID Functional Status Scale (PCFS) was administered 15 days after overcoming the infection. Age, weight, height, body mass index (BMI), and comorbidities were also taken into account. Result(s): 283 participants were included, most of them did not present any comorbidity and were on average overweight (BMI 26.88 +/- 4.97 kg/m2). Regarding the result of the PCFS scale, 74.2% did not refer any functional change or any symptoms after the disease, 11 participants manifested some kind of limitation in their daily life with a score of 2 on the scale. No participant reported severe functional limitations. (Table 1) Conclusion(s): The functionality of the healthcare workers included in the study was not affected, for the most part, it could be due to vaccination, age, and the low number of participants with comorbidities.

EFFECT OF A TELENURSING INTERVENTION IN THE CONTEXT OF THE PANDEMIC TO PREVENT CHILDHOOD ANEMIA: PILOT STUDY IN LAMBAYEQUE, PERÚ

Objective: To evaluate the effect of a telenursing intervention in the context of the pandemic to prevent childhood anemia in Peru. Material and Method: Pilot, analytical, quasi-experimental study with pre-and post-test. The sample consisted of 60 mothers from a primary health care facility in Lambayeque, Peru, who were selected at convenience and then assigned to a control group (30) and an experimental group (30), with the latter being subjected to a 4 months telenursing intervention. The effect was measured by comparing the average hemoglobin and dietary iron intake before and after the intervention. Hemoglobin level was determined biochemically through blood analysis. Dietary iron intake was collected through telephone interviews following a 24-hour recall. Results: The average hemoglobin level in the experimental group did not show significant differences between the beginning and the end of the intervention (p= 0.199);whereas in the control group, the average hemoglobin level showed significant differences between the beginning and the end of the intervention (p= 0.013). The average dietary intake of iron among the children of the control group (p= 0.049) and the experimental group (p= 0.000) had a significant difference between the beginning and the end of the intervention. Conclusion: The children who received the telenursing intervention suffered no anemia and the dietary iron intake increased, thus corroborating that telenursing programs have the potential of offering more information on nutrition, favoring the health of the child, the mother and the family. © 2022, Universidad de Concepcion. All rights reserved.

EPIDEMIOLOGICAL PROFILE OF HOSPITALIZED HUMAN METAPNEUMOVIRUS IN A CAPITAL OF CENTRAL-WEST OF BRAZIL FROM 2017 TO 2019: HIGH LETHALITY AT EXTREMES OF AGE

Human metapneumovirus (hMPV) is a paramyxovirus that causes airway infections. hMPV symptoms range from mild infections of the upper respiratory tract to infections as serious as bronchiolitis and pneumonia. From 2018 to 2019, there was a high incidence of severe acute respiratory syndrome (SARS) in the State of Goiás with a relative increase in hMPV incidence. This study aimed to assess the hMPV epidemiology of cases treated at tertiary hospitals of Goiás, as there are not significant published data from hMPV infection in Brazil. We performed a retrospective and descriptive analysis of a case series of patients infected with hMPV diagnosed by PCR (16 individuals), through medical records review from 2017 to 2019. The observed age distribution was bimodal, with the disease affecting individuals at extremes of age (median of 3.5 years old in the first stratum and median of 52 years in the second stratum). The time between the onset of flu-like symptoms and the first medical assessment had an average of 5 days. The most frequent severe symptoms were respiratory distress/dyspnea and oxygen saturation <95% (93.7% as media), even in patients without comorbidities. The most frequent complications were acute renal failure (18.7%) and healthcare-associated infections (43.7%). Death occurred in 37.5% of patients. hMPV may cause upper and lower respiratory tract infections in patients of all age groups, but the symptomatic disease occurs more frequently at extremes of age. In the pandemic caused by a new coronavirus (SARS-CoV-2), which is known to lead to influenza-like and SARS, the differential diagnosis of the etiologic agent becomes paramount. © 2022 Brazilian Society of Parasitology. All rights reserved.

Nirsevimab binding-site conservation in respiratory syncytial virus fusion glycoprotein worldwide between 1956 and 2021: an analysis of observational study sequencing data

BACKGROUND: Nirsevimab is an extended half-life monoclonal antibody to the respiratory syncytial virus (RSV) fusion protein that has been developed to protect infants for an entire RSV season. Previous studies have shown that the nirsevimab binding site is highly conserved. However, investigations of the geotemporal evolution of potential escape variants in recent (ie, 2015-2021) RSV seasons have been minimal. Here, we examine prospective RSV surveillance data to assess the geotemporal prevalence of RSV A and B, and functionally characterise the effect of the nirsevimab binding-site substitutions identified between 2015 and 2021. METHOD(S): We assessed the geotemporal prevalence of RSV A and B and nirsevimab binding-site conservation between 2015 and 2021 from three prospective RSV molecular surveillance studies (the US-based OUTSMART-RSV, the global INFORM-RSV, and a pilot study in South Africa). Nirsevimab binding-site substitutions were assessed in an RSV microneutralisation susceptibility assay. We contextualised our findings by assessing fusion-protein sequence diversity from 1956 to 2021 relative to other respiratory-virus envelope glycoproteins using RSV fusion protein sequences published in NCBI GenBank. FINDINGS: We identified 5675 RSV A and RSV B fusion protein sequences (2875 RSV A and 2800 RSV B) from the three surveillance studies (2015-2021). Nearly all (25 [100%] of 25 positions of RSV A fusion proteins and 22 [88%] of 25 positions of RSV B fusion proteins) amino acids within the nirsevimab binding site remained highly conserved between 2015 and 2021. A highly prevalent (ie, >40.0% of all sequences) nirsevimab binding-site Ile206Met:Gln209Arg RSV B polymorphism arose between 2016 and 2021. Nirsevimab neutralised a diverse set of recombinant RSV viruses, including new variants containing binding-site substitutions. RSV B variants with reduced susceptibility to nirsevimab neutralisation were detected at low frequencies (ie, prevalence <1.0%) between 2015 and 2021. We used 3626 RSV fusion-protein sequences published in NCBI GenBank between 1956 and 2021 (2024 RSV and 1602 RSV B) to show that the RSV fusion protein had lower genetic diversity than influenza haemagglutinin and SARS-CoV-2 spike proteins. INTERPRETATION: The nirsevimab binding site was highly conserved between 1956 and 2021. Nirsevimab escape variants were rare and have not increased over time. FUNDING: AstraZeneca and Sanofi.Copyright © 2023 Elsevier Ltd. All rights reserved.

Geospatial, earth observations and statistical data integration in the Cortés department, Honduras

Proper territorial data management is critical for territorial planning projects, research, innovation, and the appropriate follow-up to act for the well-being of populations. A multidisciplinary team of professionals established a pilot project named Cortes Data Hub (Centro de Datos de Cortés). It presents several dashboards that show official statistics on the energy sector, mapping the region's energy demand, data on COVID-19 cases and vaccination rates by municipality or department, and a project using Google Earth that combines post-Eta and Iota observations and a social media campaign for disaster awareness and for the promotion of activities to develop tourism in the San Manuel Municipality. This pilot project shows the importance to observe and monitor various key environmental, health, and socioeconomic data. This will help improve initiatives for local development, disaster prevention and control, and the promotion of the One Health approach. The challenges to overcome are the quality and timing of data. Training more academics, government teams, and decision-makers in the use of new tools for data integration with earth observations are important for the Cortés department's development. © 2022 IEEE.

Hazard exposure of bicyclists at urban intersections

Traditionally, mobility problems and automobile traffic congestion have increased in cities around the world due to the urban development process, especially in the city of Metropolitan Lima. For this reason, the government of the Peruvian capital has established temporary detours in certain sections of the arterial roads of the network, to invite new cyclists, due to the effects of Covid-19. Today, Lima has a network of 294 km of bicycle lanes, which have been implemented without adequate planning. In view of this, we evaluated the risk of poor planning on the vulnerable user (the cyclist) at an intersection of this road network, with a high rate of motorized congestion. The main objective of this study has been to propose corrective actions to avoid the exposure to danger on the users of the bicycle lane (countermeasures);due to lack of safety at the intersection of La Marina Ave. and Universitaria Ave. In this sense, a risk matrix was developed with the most concurrent factors that occur at this intersection;to then obtain a risk level and take actions in each of them, to mitigate the impact. The result obtained in the analysis of this study for the intersection is classified as a level 2 risk: Important risk, which means that it presents several important danger factors. Finally, in addition to the analyses developed, a treatment scheme was proposed for the intersection to provide greater safety to the users of the bicycle lane, avoiding fatal and non-fatal accidents. © 2022 IEEE.

Association of Chest CT Score During Acute COVID-19 Illness with Subsequent Lung Function Loss Among Lung Transplant Patients

Purpose: Limited data exists on CT chest abnormalities during acute Coronavirus disease 2019 (COVID-19) infection and associated post-illness loss of lung function among lung transplant (LT) patients. Method(s): The institutional database was interrogated for any LT patient diagnosed with COVID-19 during a one-year period (March 2020 to Feb 2021;n=54). 44 patients with acute COVID-19 were alive at 3-month follow up (COVID-survivors: 81.5%). Of the survivors, 34 had a CT chest during the first two weeks of acute illness. A validated CT score was used to quantify the parenchymal abnormalities due to COVID-19. Each lung was divided into 10 separate regions which were scored 0-2 based on the severity and extent of parenchymal opacification (maximum score per lung=20). To avoid confounding from underlying lung disease, only the allograph was assessed in single LT. The average score of both lungs was calculated in bilateral LT. The primary outcome measure was sustained decline of FEV1 or FVC >10% from pre-infection spirometry. Result(s): Abnormal CT score and lung opacities on CT chest were nearly ubiquitous during acute COVID-19 illness (>0;36/37, 97.3%, median score with IDR: 7.25, 4.625-10.125). The lower lobes (LL) were more affected by COVID-19 than the upper and middle lobes (UML) (median CT score in LL: 4, 2.75-6 vs 3.5, 1.25-5 in UML). A >10% decline in FEV1 or FVC was common after COVID-19 pneumonia (38.2%). The overall CT score correlated with amount of lung function loss (r=0.36, p=0.03) although the association was modest and limited to regions reflecting the UML. On ROC curve, CT score was modestly predictive of lung function decline (Fig 1). CT score from UML had the highest area under the curve (78.2%, 61.1-95.4%;p=0.006) with a score of 4.5 being the best cut-off (sensitivity 71%, specificity 85% for post-COVID lung function loss >10%). An UML CT score >4.5 was strongly associated with respiratory failure during acute illness (69% vs 24%;OR: 7.2, 1.5-33.8;p=0.01) and lung function decline >10% (77% vs 19%;OR: 14.2, 2.6-76.7;p=0.001). Conclusion(s): The CT score during acute COVID-19 infection provides prognostic information regarding loss of lung function among LT patients who survive COVID-19. Parenchymal abnormalities in the UML best predict subsequent lung function loss.

Sex-biased expression of the TLR7 gene in severe COVID-19 patients: Insights from transcriptomics and epigenomics.

There is abundant epidemiological data indicating that the incidence of severe cases of coronavirus disease (COVID-19) is significantly higher in males than females worldwide. Moreover, genetic variation at the X-chromosome linked TLR7 gene has been associated with COVID-19 severity. It has been suggested that the sex-biased incidence of COVID-19 might be related to the fact that TLR7 escapes X-chromosome inactivation during early embryogenesis in females, thus encoding a doble dose of its gene product compared to males. We analyzed TLR7 expression in two acute phase cohorts of COVID-19 patients that used two different technological platforms, one of them in a multi-tissue context including saliva, nasal, and blood samples, and a third cohort that included different post-infection timepoints of long-COVID-19 patients. We additionally explored methylation patterns of TLR7 using epigenomic data from an independent cohort of COVID-19 patients stratified by severity and sex. In line with genome-wide association studies, we provide supportive evidence indicating that TLR7 has altered CpG methylation patterns and it is consistently downregulated in males compared to females in the most severe cases of COVID-19.

Measuring the impact of remote working adaptation on employees' well-being during COVID-19: insights for innovation management environments

Purpose A new type of digital-based worker emerged during the COVID-19. As a result, during the adjustment to this scenario, family, resources and emotions were impacted. Technological and emotional skills were crucial to give continuity and certainty to business. However, despite benefits, remote working has negative consequences, especially in well-being perception. This study proposes a model to measure the impact on the well-being perception of workers that adapted their job to remote positions during a pandemic and offers a valuable framework to understand future emerging changes in remote working and the relationship with well-being perception, especially during crisis scenarios. Design/methodology/approach The study used an online questionnaire and a structural equation methodology by partial least squares (PLS) using SmartPLS 3.3.3. Data were obtained from 567 respondents workers who adapted to their jobs during the pandemic in Mexico. Findings The results showed that six model dimensions: human relations (RH), emotions (E), well-being behaviors related to Covid-19 (CB), family economics (EF), routines and habits (RS) and family life (VF) were positive and significant to reflect the Index of Perception of Well-being (iWB) using a structural equation model. This indicates how the lockdown process changed people's perception of well-being and concerns. According with this, for remote working employees, two dimensions were relevant: RH and EF. This finding is relevant because during emergency lockdown, these workers needed to adapt their activities and were separated from all human interactions. Practical implications The researchers' model of Index of Perception of Well-being (iWB) has conceptual and practical implications. From a conceptual point of view, it offers a methodology to measure the relationships between remote working and employees' well-being perception. While for practice, it offers managerial implications to better manage remote working adaptation without compromising people's well-being to create future innovation management environments (IME) for organizations. Originality/value This study contributes to develop research about changes in workers' well-being perception during digital adaptation.

Characterization of SARS-CoV-2 using the Ion AmpliSeq SARS-CoV-2 research panel

Background: The rapid spread of COVID-19 has resulted in an urgent need for effective diagnostic and therapeutic strategies against SARS-CoV-2. Next-generation sequencing (NGS) is a powerful tool in the identification and characterization of this pathogen and genomic information may aid in understanding the mechanisms of therapeutic resistance, vaccine escape, virulence, and pathogenicity. The Ion AmpliSeq SARS-CoV-2 Research Panel is a targeted NGS solution that facilitates sequence analysis of the SARS-CoV-2 genome. Paired with a bioinformatics assembly and variant calling pipelines, this assay allows for accurate characterization of the dominant SARS-CoV-2 variant. This assay's performance was analytically validated for the detection of mutations (substitutions, insertions, and deletions) in RNA derived from nasopharyngeal (NP) swabs. Method: The Ion AmpliSeq SARS-CoV-2 Research panel consists of two primer pair pools generating 237 amplicons specific to the SARS-CoV-2 virus. Reverse transcription of the RNA was performed using the SuperScript VILO cDNA Synthesis kit. Library preparation was then completed using the Ion AmpliSeq Library Kit Plus kit. The final library was quantified, normalized, pooled, and sequenced. Raw sequencing data was aligned to the AmpliSeq SARS-CoV-2 Research panel, using the MN908947.3 reference genome. Variants were called using the Torrent Variant Caller and annotated using the COVID19AnnotateSnpEff plugin. The reference-guided iterative assembler IRMA was used to produce a single consensus sequence consisting of the reference genome sequence modified to include sequence variations supported by the reads. The Pangolin COVID-19 lineage assigner software tool was used to assign SARS-CoV-2 lineage. Analytical validation was completed using controls (Twist Biosciences, BEI Resources, ATCC) and RNA derived from NP swabs. Accuracy and specificity were examined by evaluating the correctness of calling true negative variants compared to false positive and all other variant calls, respectively. Precision and limit of detection (LoD) were examined by evaluating the concordance of variants across replicate samples. Limit of Blank (LoB) was calculated as the 95th percentile of reads per amplicon in the negative samples. Results: Accuracy of base calling, specificity, and precision were 100% for SNVs, insertions, and deletions above 25% allele frequency. LoD was determined to be 576 viral copies/mL. LoB was determined to be 202 reads per amplicon. Pangolin lineage assignment was 100% for all samples. Conclusions: This panel accurately characterizes SARS-CoV-2 variants, allowing for accurate consensus sequence generation, mutation annotation, and lineage assignment.

Slowing Down Society? Theoretical Reflections on Social Deceleration in Pandemic Times (and Beyond)

The COVID-19 pandemic has caused a major change in everyday life and also reinvigorated the theoretical and political discussion on the temporal rhythms of social existence. Taking the situation of the coronavirus crisis as a starting point, the present paper attempts to provide the outlines of a theoretical account of social deceleration, a topic that has been hitherto barely explored in the field of social studies of time. In view of the complexity of the phenomenon, a distinction is made between two ways of theoretically approaching it, namely, a descriptive and a normative perspective. The paper proceeds in three steps: First, upon adopting a descriptive perspective, it advances a definition of social deceleration and proposes a typology of its different manifestations. The second step analyzes the coronavirus crisis as a process of partial deceleration of social life. The final step gives an overview of the current normative, i.e., ethical-political, disputes over social speed. © 2022 Copyright 2022 by Koninklijke Brill NV, Leiden, The Netherlands.

Comparative tolerability profile of mRNA vaccines during mass vaccination of healthcare professionals at a tertiary hospital. An active pharmacovigilance study

Background: Further understanding on the safety profile of vaccines in a real-world still need to be elucidated, such as the comparative tolerability and reactogenicity of mRNA vaccines (BNT162b2 and MRNA- 1273) beyond the controlled context of clinical trials. An active pharmacovigilance study was designed to capture a complete short-term safety profile of two mRNA vaccines BNT162b2 and mRNA- 1273, targeting incidence rates of adverse reactions within a pre-defined denominator of vaccinated healthcare professionals. Methods: A prospective active surveillance study was implemented during the vaccination campaign at Hospital Clínic by a multidisciplinary team, involving the Pharmacovigilance Technical Committee, the Department of Preventive Medicine and Epidemiology and the Department of Occupational Health Care of the Hospital. Target population of the program included vaccinated professionals from Hospital Clínic and affiliated institutions, who were vaccinated with BNT162b2 and mRNA-1273. The program was based on the delivery of a structured questionnaire by telephonic interview after each vaccine dose. A total of 64% of vaccinated professionals completed the questionnaire (n=5088). Results: A total of 85% subjects suffered at least 1 AR reaction with the vaccine. The proportion of professionals developing any AR was 2878 (81.2%) after vaccination with BNT162b2 and 1463 (92.9%) after vaccination with mRNA-1273. Severe ARs (VAS scoring ≥ 7) were reported in 1184 (33.7%) professionals after vaccination with BNT162b2 and 886 (56.4%) after mRNA-1273. In the multivariate analysis, mRNA-1273 showed a greater reactogenicity than BNT162b2 (OR=3.04 (95% CI 2.48 - 3.73;p-value: <0.0001)). Conclusions: Our study shows that mRNA-1273 has greater reactogenicity than BNT162b2. Overall, both mRNA vaccines had a reasonable tolerability profile, compared in a real-world scenario. This can be understood as a reassuring message for the medical and scientific community.

Spontaneous Intracerebral Hemorrhage in severe Covid-19 patients: Report of two cases

The respiratory system is mainly affected by the SARS-CoV-2 infection, producing a severe acute respiratory syndrome known as COVID-19 (Coronavirus disease 2019), patients with severe disease usually develop multiorgan failure;among these we can focus on the nervous system, due to its potential neurotropism. Recent clinical data reveal that patients may manifest symptoms such as anosmia, dysgeusia, impaired consciousness, headache, seizures, and cerebrovascular disease (CVD). We describe two physically active male patients with complicated SARS-CoV-2 infection without significant comorbidities related to the development of intracranial hemorrhage, nor a history of head trauma or documented anatomic malformations. Both were admitted by the emergency department and during their stay in the ICU they developed intracranial hemorrhage diagnosed by computed tomography. The paraclinical findings in the two cases were compatible with a prothrombotic state as possible etiologies of bleeding in both.